Favorite Datasets from Early (and Late) Phases of Drug Research

Hexobarbital-Metabolizing Enzyme Induction Study in Mice

Part 4 - Table 2


Fifty male and 50 female mice were assigned randomly to one of five treatment groups (10 mice of each sex/treatment group). In each sex, three groups of mice orally received one of three graded dosage levels of an investigational drug (10, 50, 250 mg/kg), one group orally received 0.9% sterile Saline (25 ml/kg) as a negative control, and one group received Sodium Phenobarbital (75 mg/kg) injected intraperitoneally. Sodium Phenobarbital was included in the experiment as a positive control since it is known to include hexobarbital metabolizing enzymes. Treatment was administered b.i.d., approximately four-and-one half hours apart, over a period of four consecutive days. A hexobarbital (100 mg/kg) injection was given intraperitoneally approximately 24 hours following the last dose of the assigned treatment. Each animal's sleeping time following hexobarbital administration was measured as the duration of time (minutes) from the loss of the righting reflex to the time of spontaneous righting. One male mouse (50 mg/kg) died from an intubation accident on the fourth day of treatment. If drug administration induces hexobarbital-metabolizing enzymes, then the length of sleep should decrease with increase in dose of drug.

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Part 4 - Table 2
Hexobarbital-Metabolizing Enzyme Induction Study in Mice
Hexobarbital Induced Sleeping Times (min.)
Males Females
Drug (mg/kg) Drug (mg/kg)
S 10 50 250 NaP S 10 50 250 NaP
21 33 32 36 9 23 19 17 20 7
29 36 33 38 9 40 23 22 24 8
32 40 35 40 9 43 24 26 28 8
37 42 37 44 10 44 27 26 29 9
38 47 40 45 10 47 32 32 31 9
41 49 41 48 10 50 34 32 33 10
42 53 44 48 13 62 38 35 35 11
45 55 49 49 13 66 40 35 37 12
51 58 53 54 14 67 40 36 45 12
56 67 (a) 60 18 67 43 51 52 14

(a) animal died


Source:

Bradstreet, T.E. and Liss, C.L. (1995) "Favorite Data Sets from Early (and Late) Phases of Drug Research - Part 4." Proceedings of the Section on Statistical Education of the American Statistical Association.

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Last modified July 24, 1997 - THS